implications for diagnosis and treatment

Inappropriately high breakpoints have resulted in systematic false-susceptible AST results to anti-TB drugs. MIC, PK/PD and clinical outcome data should be combined when setting breakpoints to minimise the emergence and spread of antimicrobial resistance. https://bit.ly/3i43wb6publishersversionpublishe

Predictive Factors and Morbidities Associated With Patent Ductus Arteriosus in Very Low Birth Weight Infants With Gestational Age 27 - 31 Weeks

A persistência de canal arterial hemodinamicamente significativo (PCAHS) é uma patologia frequente em recém-nascidos de muito baixo peso. O objectivo deste estudo foi identificar fatores de risco e morbilidades associadas à PCAHS no recém-nascido de muiot baixo peso com idade gestacional de 27 e 31 semanas. Estudaram-se os recém-nascidos(RN) com idade gestacional entre 27 e 31 semans e peso de nascimento inferior a 1500 gramas, admitidos numa unidade de cuidados intensivos neonatais entre 2010 e 2012. Realizou-se um estudo caso-coorte, tendo-se identificado os casos com diagnóstico ecográfico de PCAHS e uma amostra sistemática de RN sem diagnóstico de PCAHS (coorte de controlos). Foram explorados por regressão logística modelos preditivos da ocorrência de PACHS e da sua contribuição para a principal morbilidade neonatal. Nos três anos de estudo, a incidência de PACHS foi de 15%, intervalo de confiança (IC) 95% 11,3 - 19,5. A análise dos 44 RN com PACHS e dos 60 sem PACHS identificou como melhores preditores de PACHS a necessidade de ventilação venosa invasiva (odds ratio (OR) 3.65: IC95%1, 268 - 10,479: p=0,016) e a administração de surfatante (OR ajustado 4,52; IC95% 1,738-11,735; p=0,002); a PACHS mostrou ser significativa no modelo preditivo de leucomalácia periventricular (LPV) (OR ajustado 4,42: IC95% 1,621-12,045; p=0,004). Os resultados obtidos sugerem que estratégias preventivas e terapêuticas que permitam a redução da necessidade de administração de surfatante e de ventilação mecânica invasiva podem reduzir o risco de PCAHS. A PCAHS está positivamente associada à incidência de LPV

Effects of atmospheric conditions on surface diffuse degassing

International audienceDiffuse degassing through the soil is commonly observed in volcanic areas and monitoring of carbon dioxide flux at the surface can provide a safe and effective way to infer the state of activity of the volcanic system. Continuous measurement stations are often installed on active volcanoes such as Furnas (Azores archipelago), which features low temperature fumaroles, hot and cold CO2 rich springs, and several diffuse degassing areas. As in other volcanoes, fluxes measured at Furnas are often correlated with environmental variables, such as air temperature or barometric pressure, with daily and seasonal cycles that become more evident when gas emission is low. In this work, we study how changes in air temperature and barometric pressure may affect the gas emission through the soil. The TOUGH2 geothermal simulator was used to simulate the gas propagation through the soil as a function of fluctuating atmospheric conditions. Then, a dual parameters study was performed to assess how the rock permeability and the gas source properties affect the resulting fluxes. Numerical results are in good agreement with the observed data at Furnas, and show that atmospheric variables may cause the observed daily cycles in CO2 fluxes. The observed changes depend on soil permeability and on the pressure driving the upward flux

Impact of active efflux in the development of MDR phenotypes in Staphylococcus aureus

publishe

Control and Supervision of Wind Energy Conversion Systems

This paper is about a PhD thesis and includes the study and analysis of the performance of an onshore wind energy conversion system. First, mathematical models of a variable speed wind turbine with pitch control are studied, followed by the study of different controller types such as integer-order controllers, fractional-order controllers, fuzzy logic controllers, adaptive controllers and predictive controllers and the study of a supervisor based on finite state machines is also studied. The controllers are included in the lower level of a hierarchical structure composed by two levels whose objective is to control the electric output power around the rated power. The supervisor included at the higher level is based on finite state machines whose objective is to analyze the operational states according to the wind speed. The studied mathematical models are integrated into computer simulations for the wind energy conversion system and the obtained numerical results allow for the performance assessment of the system connected to the electric grid. The wind energy conversion system is composed by a variable speed wind turbine, a mechanical transmission system described by a two mass drive train, a gearbox, a doubly fed induction generator rotor and by a two level converter

Laurus azorica : Valorization through Its Phytochemical Study and Biological Activities

Laurus azorica (Seub.) Franco is an endemic species from the Azores, traditionally used in all the islands as a seasoning in cooking. The studies carried out with this species refer mainly to its essential oils. The study that was developed here allowed, for the first time, to determine the chemical composition and biological activities of the ethanol extract, fractions, and pure compounds from L. azorica. The hexane fraction was analyzed by GC–MS and revealed the presence of 48 compounds, comprising mainly fatty acids, fatty alcohols and terpenes, the family of fatty alcohols identified here for the first time in the genus Laurus. Three sesquiterpene lactones—costunolide, 11,13-dehydrosantonin and reynosin—were isolated for the first time in L. azorica from the same fraction, and structurally characterized using spectroscopic techniques. The compounds identified belong to families known to have relevant medicinal and nutritional properties. Regarding antioxidant activities, the results obtained showed a moderate radical scavenging effect of extracts and fractions, while in the β-carotene bleaching assay, costunolide was shown to be the most active (IC₅₀ = 4.08 ± 0.76 μg/mL), about 3.6 times more active than the standard, gallic acid, which presented IC₅₀ = 14.56 ± 0.13 μg/mL. Although the inhibition of extracellular matrix-degrading enzymes was not detected, the ethanol extract showed good inhibitory activity of tyrosinase, with an IC₅₀ of 12.04 ± 0.23 μg/mL, only 6.6-fold lower than the control kojic acid. The results presented deepen the knowledge about a little studied species, opening new perspectives for the development of value-added applications in the food and cosmeceutical fields.This research was funded by project MACBIOPEST (MAC2/1.1a/289), Interreg MAC 2014-2020, co-financed by DRCT (Azores Regional Government), as well as by FCT-Fundacao para a Ciencia e Tecnologia, the European Union, QREN, FEDER and COMPETE, through funding the cE3c center-CHANGE (UIDB/00329/2020) and the LAQV-REQUIMTE (UIDB/50006/2020).info:eu-repo/semantics/publishedVersio

ATRX, a member of the SNF2 family of helicase/ATPases, is required for chromosome alignment and meiotic spindle organization in metaphase II stage mouse oocytes

AbstractATRX is a centromeric heterochromatin binding protein belonging to the SNF2 family of helicase/ATPases with chromatin remodeling activity. Mutations in the human ATRX gene result in X-linked α-thalassaemia with mental retardation (ATRX) syndrome and correlate with changes in methylation of repetitive DNA sequences. We show here that ATRX also functions to regulate key stages of meiosis in mouse oocytes. At the germinal vesicle (GV) stage, ATRX was found associated with the perinucleolar heterochromatin rim in transcriptionally quiescent oocytes. Phosphorylation of ATRX during meiotic maturation is dependent upon calcium calmodulin kinase (CamKII) activity. Meiotic resumption also coincides with deacetylation of histone H4 at lysine 5 (H4K5 Ac) while ATRX and histone H3 methylated on lysine 9 (H3K9) remained bound to the centromeres and interstitial regions of condensing chromosomes, respectively. Inhibition of histone deacetylases (HDACs) with trichostatin A (TSA) disrupted ATRX binding to the centromeres of hyperacetylated chromosomes resulting in abnormal chromosome alignments at metaphase II (MII). Similarly, while selective ablation of ATRX by antibody microinjection and RNA interference (RNAi) had no effect on the progression of meiosis, it had severe consequences for the alignment of chromosomes on the metaphase II spindle. These results suggest that genome-wide epigenetic modifications such as global histone deacetylation are essential for the binding of ATRX to centromeric heterochromatin. Moreover, centromeric ATRX is required for correct chromosome alignment and organization of a bipolar meiotic metaphase II spindle

The Efficacy of LUCAS in Cardiac Arrest

Introduction: Chest compression is the basic technique of cardiopulmonary resuscitation (CPR) in patients with cardiac arrest. The quality and early performance of CPR is critical to improve the prognosis and chances of restoring spontaneous blood flow. Today we have manual compressions and mechanical chest compression devices. Objective: The aim of this study is to clarify the importance of the LUCAS system (The Lund University Cardiac Arrest System) in CPR. Material and methods: A systematic review of the literature by mobilizing the descriptors "Cardiac Arrest", "Lucas Efficacy" and "nursing", using the methodological head. Ten conceivable databases were selected, between 2010-2020, and seven articles were included for analysis. Results and discussion: The results of the studies are different, as four of the studies indicate that LUCAS is fully effective, particularly in fatigue and transport, but two of them do not recognise its full effectiveness. One of the studies mentions that the fact that compression by the automatic device is effective does not necessarily reflect a better result. The vast majority of the studies recognise the need for further studies to make more convincing decisions. Conclusion: Mechanical chest compression devices can improve patient outcome if used appropriately in the event of cardiac arrest. The hemodynamic performance of the LUCAS compression-decompression system is, according to some results, better than manual CPR. However, the quality of the current evidence is not sufficient. Randomised studies are needed to evaluate the effect of mechanical chest compression devices on survival inside or outsider the hospital.info:eu-repo/semantics/publishedVersio

Mycobacterium tuberculosis drug-resistance testing: challenges, recent developments and perspectives

Drug-resistance testing, or antimicrobial susceptibility testing (AST), is mandatory for Mycobacterium tuberculosis in cases of failure on standard therapy. We reviewed the different methods and techniques of phenotypic and genotypic approaches. Although multiresistant and extensively drug-resistant (MDR/XDR) tuberculosis is present worldwide, AST for M. tuberculosis (AST-MTB) is still mainly performed according to the resources available rather than the drug-resistance rates. Phenotypic methods, i.e. culture-based AST, are commonly used in high-income countries to confirm susceptibility of new cases of tuberculosis. They are also used to detect resistance in tuberculosis cases with risk factors, in combination with genotypic tests. In low-income countries, genotypic methods screening hot-spot mutations known to confer resistance were found to be easier to perform because they avoid the culture and biosafety constraint. Given that genotypic tests can rapidly detect the prominent mechanisms of resistance, such as the rpoB mutation for rifampicin resistance, we are facing new challenges with the observation of false-resistance (mutations not conferring resistance) and false-susceptibility (mutations different from the common mechanism) results. Phenotypic and genotypic approaches are therefore complementary for obtaining a high sensitivity and specificity for detecting drug resistances and susceptibilities to accurately predict MDR/XDR cure and to gather relevant data for resistance surveillance. Although AST-MTB was established in the 1960s, there is no consensus reference method for MIC determination against which the numerous AST-MTB techniques can be compared. This information is necessary for assessing in vitro activity and setting breakpoints for future anti-tuberculosis agents